Friday, November 29, 2019
Abortion Essays (1661 words) - Abortion, Free Essays, Term Papers
Abortion Essays (1661 words) - Abortion, Free Essays, Term Papers Abortion Abortion is the ending of pregnancy before birth and is morally wrong. An abortion results in the death of an embryo or a foetus. Abortion destroys the lives of helpless, innocent children and illegal in many countries. By aborting these unborn infants, humans are hurting themselves; they are not allowing themselves to meet these new identities and unique personalities. Abortion is very simply wrong. Everyone is raised knowing the difference between right and wrong. Murder is wrong, so why is not abortion? People argue that it is not murder if the child is unborn. Abortion is murder since the foetus being destroyed is living, breathing and moving. Why is it that if an infant is destroyed a month before the birth, there is no problem, but if killed a month after birth, this is inhumane murder? It is morally and strategically foolish, because we lose the middle when we talk about reproductive rights without reference to a larger moral and spiritual dimension, and we are unwilling to use language like transgression and redemption, or right and wrong. -Wolf p54 The main purpose abortions are immoral is how they are so viciously done. Everyday, innocent, harmless foetuses that could soon be laughing children are being brutally destroyed. One form of abortion is to cut the foetus into pieces with serrated forceps before being removed, piece by piece from the uterus by suction with a vacuum aspirator. Another form consists of bringing the foetus feet first into the birth canal, puncturing its skull with a sharp instrument and sucking out the brain tissue. The body parts, such as the head, are given letters, rather than refer to the parts as what they are. In my opinion this is for the doctors who cannot face the reality of what they are doing. The remains of the foetus or embryo, as the case may be, are put into everyday, plastic buckets and then sent to a dumpster where these precious bones and limbs are disposed. However, how and when an abortion takes place are matters of little importance to pro- abortionists and other defenders. Even former abortion practitioners from varying backgrounds and religions have a new view on abortion. These changes of heart were caused by psychological, religious and scientific reasons. One doctor, Dr. Bernard Nathanson, performed 60,000 abortions and supervised 10,000, before scientific evidence and the use of an ultrasound, convinced him he was promoting and participating what he now calls the most atrocious holocaust in the history of the United States. Other doctors refuse to perform legal abortions, saying they should save lives rather than destroy them. Many argue is it the women's or the foetus' rights and values that are being trampled on? Pro-choice movements sometimes fall back on an abortion rhetoric that seems to dehumanize and trivialize the death of a foetus as a way to humanize and make important the reproductive rights of women. (Wolf p54) Women can treat an unwanted foetus as a violation of her civil rights and is therefor justified tin using force to expel it (McMillan pA12) The decision is not up to the mother because she is not God. Only God, the ultimate creator has the right to choose who may live and who shall die. Humans do not have the right or the power to control the quality of life and to avoid suffering. The issue of abortion is not just life, but how life is created and the extent to which human intention and control the process, both before and after birth. All humans inventions and interventions may give us a world to regret. (Clark p3) With abortion, we humans give ourselves dominion over a large part of God's plans and our destiny. Abortion becomes especially evil when the bond between mother and child is broken and it is being used as an alternative birth control when humans cannot control their irresponsible sexual hungers. If beings are responsible enough to be sexually active, they should also be responsible enough to accept consequences, and if that means becoming pregnant and creating a life, then that life should have the opportunity to live. There should be a bond or relationship between a mother and child, whether born or unborn. Mothers and her children form a bond unlike any other felling of love; when a child is aborted, before given the right to grow in the bond, does the mother feel the connection with her child or is it just uterine material. Abortion is never about just abortion. It is
Monday, November 25, 2019
Lying Explored in Cat on a Hot Tin Roof essays
Lying Explored in Cat on a Hot Tin Roof essays "Mendacity is a system that we live in. Liquor is one way out and death's the other."[1] This quote by Tennessee Williams is expressed throughout one of his best-known works, Cat on a Hot Tin Roof, and the theme of mendacity permeates the entire play. Mendacity is a term that refers to lies, hypocrisy and deception that the Pollitt family uses to escape from falsehood. The overburdened circumstances of the family crisis reveal hidden truths that were being held by the characters in the play. Brick drinks in order to escape mendacity and lies, the Pollitt family, except Brick lies to Big Daddy about his terminal cancer and Big Daddy himself is Brick drinks in order to escape mendacity and lies. The alcohol helps him cope with issues he has bottled up inside and eases the pain he inflicts on himself by denying the nature of his relationship with Skipper and his culpability in Skippers self-destruction and death. Brick confesses to Maggie that alcohol is the only way he can obtain peace of mind and says, "The click in my head when I've had enough of this stuff to make me peaceful... (1.33)" Brick feels responsible for Skipper's suicide because he rejected him after he confessed his feelings for Brick. During his first real discussion with Big Daddy, Brick spits out his disgust with mendacity. He is repulsed with the fake life he has been living when his friendship with recently deceased Skipper was misinterpreted as "dirty". However, Big Daddy states that Brick's disgust with mendacity is really disgust with Uh-hu. Anyhow now!-we have tracked down the lie with which you're disgusted and which you are drinking to kill your disgust with, Brick. You been passing the buck. This disgust with mendacity is disgust with yourself. You!-dug the grave of your friend and kicked him in it!- before you'd face truth with him! (2.127) Big Daddy genuinely lov...
Thursday, November 21, 2019
International and National Relief Efforts - BHS412 Module 4 - SLP Essay
International and National Relief Efforts - BHS412 Module 4 - SLP - Essay Example For this particular Catastrophic Disaster Response Plan, the idea was to keep all databases and information updated on an ongoing basis. What this translates to is that all maintenance and training will have to be constantly updated and modified to adapt to the situation at hand as well as predicted future incidents. For example, it could be the case that one particular disaster relief scenario takes place in an area in which there is very little or no water available to disaster affected people, and in another situation there would be an abundance of water. Given the former scenario, staff involved would have to have special training as to steps that can be taken to ensure that water arrives as fast as possible to affected people wherein in the later scenario (Assuming available water is not polluted) acquisition of water could take a back seat to more important products and or services. In effect all training will have to be undertaken on an ongoing basis and will be based upon new developments in technology. The possible strengths of the CDRP are fairly obvious insofar as all people contributing to various disaster relief efforts would have already received training on numerous scenarios and would have effectively had exposure with all of the latest technologies.
Wednesday, November 20, 2019
Online Course Materials and Online Educational Tools Essay
Online Course Materials and Online Educational Tools - Essay Example Some courses are therefore available through a face to face teacher interaction which is supplemented by online course material and other educational tools. There has been some debate about the effectiveness of the online courses per se or of the supplemental course materials to add to the studentsââ¬â¢ understanding of the subject (Allen and Seaman, 2006) .Moreover, previous research has also found that teachersââ¬â¢ perceptions about the effectiveness of the online course or course material differs vastly (Dawley, 2007) which may in turn impact on their enthusiasm to adopt their teaching to the new requirements entailed by the online mode of learning. This research therefore aims to assess what teachers in college X at the University of Y, in Jeddah, KSA think about the online course material that the university uses to supplement the classroom teaching. As such, the main research questions are: 1. What do the teachers think are the advantages and disadvantages of the online course material? 2. What are some of the barriers and challenges that teachers face while developing and managing the online content for their courses? 2. Literature Review According to Allen and Seaman (2006), online education is not restricted to the fringe anymore instead the popularity of online education is increasing rapidly (Dawley, 2007). In fact, it has become crucial to the long term plans of any institution to incorporate online courses for both local and international students. As more and more teachers begin to adapt to this method of teaching, it is necessary to understand their perceptions about moving to an online approach. This paper takes at look at the perception college teachers have about online... As the report declares two teachers are interviewed from a University in Jeddah, and their opinion sought on the impact on student learning that the online course content that the University uses to supplement regular classes. The teachers are selected on the basis of convenience sampling from among the acquaintances of the researcher. The interviews are conducted over Skype, and the responses are analysed in a reflective manner. The findings are discussed to highlight the advantages and disadvantages of the online course content, and recommendations made for the improvement of the same.This paper highlights that online education is not restricted to the fringe anymore instead the popularity of online education is increasing rapidly. In fact, it has become crucial to the long term plans of any institution to incorporate online courses for both local and international students. As more and more teachers begin to adapt to this method of teaching, it is necessary to understand their per ceptions about moving to an online approach. This paper takes at look at the perception college teachers have about online course materials and online educational tools.à Many researchers have made the observation that in spite of the high demand of online courses, faculty members are not very keen to shift their mode of teaching to an online medium.à One of the major concerns of adopting an online approach is pedagogical ââ¬â whether the process of teaching would be as effective as that of a classroom.
Monday, November 18, 2019
Operation Analysis Essay Example | Topics and Well Written Essays - 500 words
Operation Analysis - Essay Example Corporate contract bookings were also increased as a result. The average revenue earned by the Business and corporate segment together amounted to 40% of total revenue in Year 1. The percentage remained constant in year 1, and the same two segments with average revenue earnings of 38% approx. also support the current revenue. The company, however, is striving hard in the marketing department to increase its leisure standard and premium sales that are expected to be increased in July due to holidays and successful marketing campaigns. Total revenues of the company were observed to be lowest in February this year with $0.8 million. This also had a negative impact on cash flows of the company. However, the subsequent increase in advertisement expenditures and holidayââ¬â¢s factor, the revenues were reported in this month to be equal to $1.1 million approx. with a net profit of $0.18 million. Departmental financial analysis revealed that departmental profits of the company had increased over time and this monthââ¬â¢s departmental profits were reported to be 62% as compared to 54% last year. The growth in profit percentage was due to the cost control measures adopted in the food and beverages expenses, which successfully resulted in the total expenses to be 68% as compared to 95% in the last year. The room services in which profits net of departmental expense were 45% earned major revenues of the company. Cost control measures were also adopted in the administrative and general expenses, which resulted in a decrease of expenses to 12% as compared to 17% last year. Marketing and sales, however, were increased slightly due to the advertisement campaigns and discounts offered by the company to increase the summer sales packages. Fixed expenses of the company were reported to be performing positively, and the positive decline in total fixed expenses was observed. Fixed expenses this month were 13.4% as against 18.4% reported in
Saturday, November 16, 2019
Nightmare Before Christmas | Analysis
Nightmare Before Christmas | Analysis Tim Burton rings in my ears as one of Hollywoods most eccentric directors. There is a distinct and unique phenomenon in his films: the genre of his most films are between animation films and real person films; his animation films overturn the world of traditional fairy tales, showing the darkness of horror, but did not give us the feel of fear. Nightmare before Christmas, which reflects his typical paradigm, is a fantasy mixed of horror, magic, darkness, holidays, the jolly guys, and an interesting skeleton. The features of Nightmare before Christmas have a similarity with Bakhtins theory of carnivalesque. In Bakhtins view, everyone lives a carnivalistic life as long as those carnival laws. His feel of carnival is full of grotesque realism, which can get rid of the horrable things in the world. And the grotesque style can also turn the world into a bright and cheerful one. (Bakhtin,1989). There is always a feel of carnival surrounded in Nightmare before Christmas. Because of the feel, audience can get away from the fear successfully. On the other hand, Bakhtin believes that carnivalistic life is a life drawing out of its usual rut; it is to some extent life turned inside out, the reverse side of the world.( Bakhtin,1989) Similarly, Burton sets two worlds to make a comparision: the human world and underground world; the grotesque world of Halloween and the happy world of Christmas. The confrontation of the two worlds represents Burtons inclination. The happy world is boring while the eerie wolrd is vivid and interesting. In my Nightmare before Christmas lingers on the edge of conventional culture and popular culture, it challenges the conventional culture and queries on the popular culture. So Burton makes an utmost effort to prove the rationality of his subversion in this film. It takes delight in talking about Burtons Gothic darkness of horror. The story happens in the world of ghosts and monsters where we are totally un familiar. The vampire bats, red eye demon , black monsters and even the wretched green, these gothic images are everywhere in the film. They stand in stark contrast to the simplicity of traditional animation films. However, the audience feel more closer to them and come to realize their subsistence status trenchantly. The American animation films have a heroism complex, but Burton deconstructs our expectation of the traditional hero. In Nightmare before Christmas, the character Jack is frightful and he has no mission to save the world. Other character is treated equally, eg. the pet comes along with Jack is soul of a dog instead of a brilliant white horse. More importantly, Burton wants to create a balance between creepy and interesting. So he gives life to the grotesquery Jack who actually is virtuous. He demonstrates the characters individual emotions and action which has no relationship with hero. Specificly, the modeling of Jacks eyes are just two black holes which thoroughly give expression to the meaning of the character. Lets turn to the other film: No Country for Old Men In this film No Country for Old Men the genre pushes the envelope of sanity. It is an unconventional western film, mixed with action, crime drama and dark humor. The McCarthys novel No Country for Old Men is an ideal vehicle for the Coen brothers, who have used violence and emptiness laced with dark humor as an artistic aesthetic in their directing careers. The films greatest achievement is its ability to transport the audience into a world where the predestination, death, fears, choices, morals and realities of life are strikingly brought to life and make the audience reflect upon and look back with careful consideration after the whole experience. The violence and bleakness of it all is not to simply evoke reaction or engage the audience, it is to tell a story and impart an experience of great intention, to which the Coens have brilliantly succeeded. To some extent, this film is a character study of Sheriff Bell, an honest lawman who is wise, observant, grounded in reality, and has a long memory. No Country for Old Men is really his story. And also, The Coen brothers direction of the particularly intense chase scenes between Chigurgh and Moss are masterful, evoking emotions of suspense to the highest level and pushing the audience to the very edge of their seats. At the same time the movie is a character study on the effects of evil and innocence lost, an exploration on the themes of fate and chance, an analysis of the freedom to choose and its consequences, a reflection on evil and good as forces of society and the investigation of basic human emotions such as hope, fear, love, violence and aspiration in the face of a variety of situations. The ending of the film is about death. Faced with the overwhelming fact of death, all of us humans find meaning only by making up their own spiritual codes. The murderers code, the good old boys code, the sheriffs code, the wifes codethey are all equally meaningless, equally inadequate in the face of death, but they are all perfectly legitimate responses to the fact of universal death. The film is also about predestination and free will, Chigurgh doesnt believe in free will, I got here the same way the coin did , he is almost like a robot , and scientifically he is totally right we dont have free will , Chigurgh is not guilty , he just has killer genes and his mother didnt hug him so thats why he turned out to be this way, there are no choices in matter , just cause and effect , only quantum psychics can show some difference. In conclusion, Film genres can take culture as very important elements into consideration. And also, we can characterize a culture by its genre set. Obviously through the film review, we can see that genre can function as a tool to define and describe a culture. Heroes and Villains: Historical Analysis Heroes and Villains: Historical Analysis Heroes, Villains or Both? Austin Rappel What I found most interesting in this weeks readings was the myths behind the men; specifically Ulysses S. Grant, general in chief of the United States Army, William T. Sherman, general in The United States Army and Abraham Lincoln, president of the United States. Grant was considered a drunk, Whatever happened, the story of his drinking became a staple of gossip in the old army. (McPherson, pg.114) Sherman was considered crazy; But Sherman could never entirely escape the reputation of madness (McPherson, pg 114) Lincoln was considered passive: a basic trait of character evident throughout Lincolns life: the essential passivity of his nature. (McPherson, pg. 206) I think it is important to determine if these things were true or if there is enough evidence to ever know for sure the mindset or character of the men that are known to us as the men that defined and ultimately were the victors of the Civil War. Grant quickly rose through the ranks during the Civil War; command of a brigade, a division, an army (Army of Tennessee), an army group, an all of the armies of the United States. (McPherson, pg 110) These accomplishments are a big contrast to what one could consider could be an accomplished by a drunkard. Brooks Simpson , a biographer concluded, Although Grant sometimes took a drink during the war, and may on occasion have taken two, his colleagues who knew him best and were in the best position to observe him were unanimous in their testimony that he was rarely if ever drunk. (McPherson, pg. 114) Grant was also given the reputation as a butcher when the general most deserving of the title was Robert E. Lee. (McPherson, pg 113) Sherman is best known for his March to the Sea; a march of over 700 miles, in which psychological warfare was used in order to stop the war. As stated in McPherson, The terror that his soldiers provoked among Southern whites was a power, he wrote, and I intended to utilize it to humble their pride, to follow them to their inmost recesses, and to make them fear and dread usà ¢Ã¢â ¬Ã ¦ We cannot change the hearts and minds of those people of the South, but we can make war so terrible and make them so sick of war that generations would pass away before they would again appeal to it.(McPherson, pg. 124) As stated in the article by Matt Carr, General Shermans March to the Sea, Shermans plan was to attack the infrastructure of the south and therefore end the war; After more than three years of violent and seemingly endless conflict, Sherman had decided to take the conflict beyond the battlefield and subject Georgia to a level of devastation that would make its population realise that w ar and ruin are synonymous terms. (pg. 30) Its hard to imagine that these strategies were those of a crazy man. Lincoln, the 16th president of the United States and the author of many documents most notably the Emancipation Proclamation was seen at least by one biographer, David Herbert Donald, to be passive. However, as stated in McPherson, at the very outset of his presidency Lincoln demonstrated traits that were the opposite of what Donald calls his essentially passive personality. (McPherson, pg. 207) Its hard to believe that the man that set into motion the Civil War and wrote one of our most historical documents has a passive personality. Maybe the passivity noted by some is more a compassion than anything else. As stated in, Lincolns Legacy, Lincoln Lives Through His Words by Gail Fineberg, Lincolns first draft of the Emancipation Proclamation, which he presented to his cabinet on July 22, 1862. Nobody liked it Sellers said. Lincoln came back to his cabinet with a second draft in September, and admonished members to comment not on substance but on his style. (pg 38) The quote goes witho ut saying to the mindset of a determined man more so to the mindset of a passive man. Although it may never be known the actual character of Grant, Sherman orà Lincoln, it will be left up to the readers and historians perceptions of whether are not these men were heroes, villains or maybe a little of both during and after the Civil War. It goes without saying that these men, however viewed, will always be remembered for the mark they left on the Civil War and wars to come. Polymerase Chain Reaction (PCR): Optimization Parameters Polymerase Chain Reaction (PCR): Optimization Parameters IGHODARO OGHOGHO UYIOSA Polymerase chain reaction (PCR): Evaluation of different optimization parameters for appropriate PCR process. Abstract PCR is a method used to produce relatively large amounts of a specific DNA sequence. The productivity of PCR method depends on different reaction conditions such as the concentration of the DNA template, concentration of magnesium ions, DNA template dilution and polymerase concentration. The aim of this study was to find optimal reaction conditions required for appropriate PCR process. To check the correct conditions the agarose gel and polyacrylamide gel electrophoresis were used. One single, strong amplified band and no unspecific product describe the most suitable amount of given conditions. In the results of this experiment, the most suitable annealing temperature was 62oC, the most proper amount of concentration of magnesium was 2nMol, and the best template dilution was 2à µl. The important parameters evaluated in this experiment were template optimisation, annealing temperatures and magnesium chloride concentrations with and without touchdown PCR. Introduction PCR is a simple, enzymatic assay, which allows for the amplification of a specific DNA fragment from a complex pool of DNA. PCR can be done using source DNA from a variety of tissues and organisms, including peripheral blood, skin, hair, saliva, and microbes. Only trace amounts of DNA are needed for PCR to generate enough copies to be analysed using conventional laboratory methods. For this reason, PCR is a sensitive assay (Lilit andNidhi 2013). To carry out a PCR it is essential to have the following reagents: DNA template, forward and reverse primers, PCR buffer, magnesium chloride (MgCl2), dNTP and DNA Taq polymerase. The annealing temperature allows for the annealing of the primers to the single stranded DNA. It depends on the length and composition of the primers. If the temperature is too high, then the primers will not anneal correctly, and if the annealing temperature is too low then the primers will anneal non-specifically (Hecker et al. 1996). Magnesium ions interact with the DNA polymerase enzyme during this process. The magnesium ion interacts with negatively charged molecules in the reaction. Positive ions of magnesium interact with the negatively charged DNA strands to mask the forces of repulsion (Markoulatos et al. 2002). Template DNA is a fragment of DNA which is needed to create required copies. DNA Taq polymerase is a polymerase enzyme, which is essential for DNA replication, this means that DNA polymerase synthesizes DNA molecules from their nucleotide building blocks (Huang et al. 1992). The nucleotides include the four bases ââ¬â adenine, thymine, cytosine, and guanine (A, T, C, G) ââ¬â that are found in DNA. These act as the building blocks that are used by the DNA polymerase to create the resultant PCR product. During this experiment, two methods were used to visualise the PCR products formed. These were Polyacrylamide gel electrophoresis (PAGE) and Agarose gel electrophoresis method. Electrophoresis is a separation procedure which is based on the separation of DNA fragments by size, shape and charge. The clue of this process is the mobility of ions in an electric field (nucleoid acids, which are negatively charged migrate to the anode ââ¬â positive electrode) (Stellwagen, 1998). Aim The aim of this experiment was to assess which parameters as annealing temperature, concentration of magnesium, and template DNA influence DNA amplification efficiency and specificity. 2.0à Materials and methods This section was divided into two parts. In part A, a PCR reaction 1 set-up using optimised PCR mastermix was done, while in part B, a PCR reaction was set-up to test four different variables to optimise a PCR reaction. 2.1à Part A Setting up the PCR reaction A mastermix enough for four reactions was made. 30à µl of H2O, 50à µl of 2X PCR mastermix and 4à µl each of forward and reverse primers were pipetted and mixed in an Eppendorf tube from which, 24à µl of the mastermix was pipetted into three separate PCR tubes (i.e. tube 1, 2, and 3). 1à µl of sample DNA, 1à µl of 1/10 diluted DNA and 1à µl of H2O were added to each tubes respectively and each amplified on a PCR block running the following programme: Denaturing step done at 94oC for two minutes Amplification step done at 94oC for 30 seconds, 55oC for 30 seconds and 72oC for 1 minute. This step was repeated for 35 cycles. Finally, the extension step was done at 72oC for 3 minutes. Agarose gel electrophoresis (2% agarose gel for PCR) An agarose gel was submerged in a gel tank filled with TBE buffer. Then 5à µl of gel loading buffer was added to each sample and mixed. Next, the first well was loaded with the molecular weight marker and then 10à µl of each sample was loaded into each respective wells. Next, the gel was run for 45 minutes at 80V. Finally, the gel was visualised on the gel documentation system to show separation and migration of the DNA. 2.2à Part B PCR optimisation reaction For this protocol, PCR reactions were set up and individual components of the reactions were varied in other to optimise them as follows: Annealing temperature optimisation 17.75à µl of water, 2.5à µl of 10x reaction buffer, 0.75à µl of Magnesium Chloride (50mMol), 0.5à µl of dNTPs, 1à µl each of forward and reverse primers, 0.5à µl of Taq polymerase and 1à µl of DNA were pipetted into 5 separate tubes (i.e. tube 1, 2, 3, 4, 5) which were then placed on a gradient PCR block, with one tube at each of the following temperature 46oC, 52oC, 55oC, 58oC, and 65oC. Next, the PCR block was set to the following programme: Denaturing step at 94oC for two minutes Amplification step at 94oC for thirty seconds, 46-65oC for thirty seconds, and 72oC for one minute. This stage was repeated for thirty-five cycles. Finally, the extension step was done at 72oC for three minutes. Template optimisation A PCR mastermix containing 13.75à µl of water, 2.5à µl of 10x reaction buffer, 0.75à µl of 50mMol magnesium chloride, 0.5à µl dNTPs, 1à µl each of forward and reverse primer and 0.5à µl of Taq polymerase were pipetted into five tubes. Next, 5, 2, 1, 0.1, and 0.01à µl of template DNA and, 0, 3, 4, 4.9. 4.99à µl of H2O were added to each tubes respectively. Next, the tubes were then amplified on a PCR block using the same cycle parameters as set out in part A. Magnesium concentration A PCR mastermix mastermix containing 16à µl of water, 2.5à µl of 10x reaction buffer, 0.5à µl dNTPs, 1à µl each of forward and reverse primer, 0.5à µl of Taq polymerase and 1à µl of DNA were pipetted into five tubes (i.e. tube 1, 2, 3, 4, and 5). Next, a Mgcl2 dilution was prepared to get a final Mgcl2 mMol of 0.5, 1.5, 2, 3 and 5mMol which were then added to each tubes respectively to give a final volume of 25à µl. Next, the tubes were then amplified on a PCR block using the same cycle parameters as set out in part A. Touchdown PCR A PCR mastermix was prepared using the same variables outlined for magnesium concentration. Then the touchdown PCR programme used was as follows: 94à °C for 3 minutes then 94à °C for 30 seconds, 64à °C for 30 seconds and 72à °C for one minute for three cycles, 94à °C for 30 seconds, 61à °C for 30 seconds, and 72à °C for one minute for three cycles, 94à °C for 30 seconds, 58à °C for 30 seconds, and 72à °C for one minute for three cycles, 94à °C for 30 seconds, 55à °C for 30 seconds, and 72à °C for one minute for three cycles, 94à °C for 30 seconds, 53à °C for 30 seconds, and 72à °C for one minute for thirty cycles, and finally 72à °C for three minutes. Sample preparation and polyacrylamide gel electrophoresis After all the different optimisation protocols, the samples to be loaded into the gel were prepared by adding 5à µl of loading buffer to each PCR reaction and mixed. Next, 5à µl of DNA ladder was pipetted into the first well while 10à µl of sample were pipetted into each designated wells. The gel was then run at 100V for 45 minutes. After which, the gel was recovered and placed in a weighing boat containing 1x TBE buffer. Next, 5à µl of ethidium bromide was carefully introduced into the weighing boat and left for 10 minutes before visualisation with a gel documentation system. 3.0à Result PCR reactions were set up in 5 different PCR tubes with all the required components for complete PCR reaction , save for a particular factor which was varied to ascertain the optimal concentration necessary for the production of the highest amount of pure specific product. The results obtained from these various optimised factors are represented in the gels below. Part A Optimized PCR 1à µl of DNA template was loaded in the well labelled neat DNA and it revealed the highest amount of product formed, the well containing a 1/10 diluted DNA had a much lower amount of product formed while the well with the water blank yielded no product. Also, unspecific products were not formed. Figure 1: Optimised PCR agarose gel. Part B The results of different PCR optimisation reactions PCR annealing temperature optimisation Figure 2: PCR annealing temperature optimisation polyacrylamide gel. Key: L-molecular weight ladder, lane 1- 48à °c, lane 2- 52à °c, lane 3- 55à °c, lane 4- 62à °c, lane 5- 65à °c, unspecific product, specific product. From Figure 2 above, there are three unspecific products formed in lane 1, a greater specific product with insignificant unspecific product formed in lane 2, insignificant unspecific products formed in lane 3 and there is also a decrease in the intensity of the specific product formed compared with that of lane 2. While in lane 4, there is a minor decrease in the intensity of the specific product formed thus, the intense quantity of the specific product formed (i.e. there is a strong amplification here) and in lane 5, there is a major decrease in the quantity of specific product formed. Therefore, the quantity of the product decreases as the quantity of the template DNA decreases, and the quantity of the specific and unspecific products increases as the quantity of the template DNA increase. Template dilution optimisation Figure 3: Template dilution optimisation polyacrylamide gel. Key: L -molecular weight ladder, lane 1- 5à µl, lane 2- 2à µl, lane 3- 1à µl, lane 4- 0.1à µl, lane 5- 0.01à µl template DNA, specific product, unspecific product. From the gel above, there are three unspecific products formed in lane 1 and also the intensity of the specific product formed is high, the unspecific product formed in lane 2 is insignificant and the specific product formed is greater in intensity (i.e. has a higher amplification) compared with that of lane 1, in lane 3 and 4, the intensity of the specific product formed decreased compared with that of lane 2, while in lane 5, there is a major decrease in the intensity of the specific product formed. Therefore, the quantity of the product decreases as the quantity of the template DNA decreases, and the quantity of the specific and unspecific products increases as the quantity of the template DNA increase. Magnesium dilution optimisation Figure 4: Magnesium dilution optimisation polyacrylamide gel. Key: L -molecular weight ladder, lane 1- 0.5mmol, lane 2- 1.5mmol, lane 3- 2mmol, lane 4- 3mmol, lane 5- 5mmol, specific product, unspecific product. Form the gel above, there is an unspecific and a specific product formed in lane 1, in lane 2 there is a decrease in the intensity of the unspecific product formed and also there is an increase in the amount of specific product formed. In lane 3, there were no production of unspecific products and the intensity of the specific product formed remained high. In lane 4, there is evidence of the presence of an unspecific product formed but the intensity of the specific product formed remained high. While in lane 5, there is a minor reduction in the intensity of the specific product formed while there is visible presence of formation of unspecific products. Magnesium touchdown optimisation Figure 5: ââ¬âTouchdown magnesium concentration optimisation. Key: L ââ¬â molecular weight ladder, lane 1- 0.5 mMol, lane 2- 1.5 mMol, lane 3- 2mMol, lane 4- 3 mMol, lane 5- 5mMol, unspecific product, specific product. From the gel above, in lane 1, the intensity of the unspecific product formed is the same with that of the specific product formed. While in lane 2, 3 and 4, the intensity of the specific products formed are the same while the intensity of the unspecific products gradually decreased. There was no unspecific product formed in lane 5, however there was a reduction in the intensity of the specific product formed compared to that of lane 4. Discussion This experiment was performed to evaluate different optimisation protocols to optimise PCR reactions. For the PCR reaction using an optimised PCR mastermix in part A, the highest amount of products formed was observed in the well containing 1à µl of DNA template.The annealing temperature is the most important optimisation, because it can have an influence on the specificity of the reaction. If the temperature is too high, the hybridization will not take place thus, templates and primers remain dissociated. If the temperature is too low, mismatched hybrids will occur. Correct annealing temperature must be low enough to start hybridization between template and primer, and also high enough to prevent forming mismatched hybrids (Roux, 2009). According to the results from annealing temperature optimization polyacrylamide gel (Figure 2), the most suitable annealing temperature was 62oC, because the band was clear and single as opposed to the 48oC, 52oC and 55oC, where the smears (i.e. unspecific products) were shown. The intensity of the band in 62oC was the strongest compared with that o f 65oC. The most suitable template dilution for PCR was 2à µl, because it gave in the polyacrylamide gel in Figure 3, the most bright, single band with very low amount of unspecific products formed. Besides, annealing temperature and template dilution parameters, PCR reaction components could also lead to non-specific amplification. Two variables, which are reported to greatly influence the specificity of the PCR reaction, are magnesium and dNTP concentration (Dwivedi et al. 2003). For magnesium dilution touchdown (Figure 5), the molarities at 1.5, 2 and 3mMol showed very similar amount of products formed indicating the importance of the magnesium in PCR amplification while for magnesium dilution without touchdown (Figure 4), it was found that 2mM yielded the best results. Magnesium concentration is known to play a critical role in amplification as it can affect DNA strand denaturation, primer annealing specificity and enzyme fidelity. These observations are in agreement with earlier studies (Innis et al. 1990; Eeles et al. 1993). Even brief incubations of a PCR mix at temperatures significantly below theTmcan result in primer-dimer formation and nonspecific priming. Hot-start PCR methods (Erlich et al. 1991;Ruano et al. 1992) can dramatically reduce these problems. In this experiment, two methods were used to visualise the PCR products formed. They are, Polyacrylamide gel electrophoresis (PAGE) and Agarose gel electrophoresis. Agarose gel is the most popular medium for the separation of moderate and large-sized nucleic acids and have a wide range of separation but a low resolving power, since the bands formed in the gels tend to be indistinct and spread apart. This is a result of pore size and cannot be largely controlled (Stellwagen, 1998). However, Polyacrylamide gels are normally more difficult to prepare and handle, and it requires a longer time for preparation than agarose gels. However, polyacrylamide gels have a greater resolving power, can accommodate larger quantities of DNA without any significant loss in resolution and the DNA obtained from polyacrylamide gels is extremely pure (Guilliatt, 2002). Hence, they are better than agarose gels. It should be noted that polyacrylamide is a neurotoxin (when unpolymerized), but with proper labo ratory care it is no more dangerous than various commonly used chemicals in the laboratory (Budowle Allen, 1991). Conclusion Optimisation of Polymerase Chain Reaction is very important for PCR performance to minimize failures, avoid the production of non-specific products and increase specificity of the reaction. The knowledge of proper conditions allows to use PCR correctly and to receive good results. According to the results of the research the most suitable annealing temperature was 62oC, the most proper amount of concentration of magnesium was 2nMol, and the best template dilution was 2à µl. Those parameters give DNA amplification specificity and efficiency. (Harris and Jones, 1997). References Alka, D., Sarin, B., Mittar, D., Sehajpal, P. (2003). OPTIMIZATION OF 38 kDa BASED PCR ASSAY FOR DETECTION OF MYCOBACTERIUM TUBERCULOSIS FROM CLINICAL SAMPLES. Journal of Tuberculosis. 50:209-213. Budowle, B. and Allen, R. (1991). Discontinuous polyacrylamide gel electrophoresis of DNA fragments. Methods in Molecular Biology. 9:123-132. Eeles, R. and Stamps, A. (1993). Managing the method. In Polymerase Chain Reaction (PCR) the Technique and its Application. Journal of Applied Sciences Research. 2(3): 12-26. Erlich,H.,Gelfand,D.,Sninsky,J. (1991).Recent advances in the polymerase chain reaction.Science.252:1643ââ¬â1651. Guilliat, A. (2002). Agarose and polyacrylamide gel electrophoresis: PCR mutation detection protocols. Methods in Molecular Biology. 187:125-137. Hecker,K. and Roux,K. (1996).High and low annealing temperatures increase both specificity and yield in touchdown and stepdown PCR.Bio Techniques. 20:478ââ¬â485. Harris, S. and Jones, D. (1997). Optimisation of the polymerase chain reaction. Journal of Biomedical Science. 54 (3):166-173. Huang, M., Arnheim, N., Goodman, M. (1992). Extension of base mispairs by Taq DNA polymerase: implications for single nucleotide discrimination in PCR.Nucleic Acids Research.20 (17):4567ââ¬â4573. Innis, M. and Gelfland, D. (1990). Optimization of PCRââ¬â¢s. In PCR protocols: A guide to methods and applications. Indian Journal of Tuberculosis. 118:1589-1599. Markoulatos, P., Siafakas, N., Moncany, M. (2002). Multiplex polymerase chain reaction: a practical approach.Journal of Clinical Laboratory Analysis.16(1): 47ââ¬â51. Lilit, G.andNidhi, A. (2013). Research Techniques Made Simple: Polymerase Chain Reaction (PCR). Journal of Investigative Dermatology. 133 (3): 4565-4579. Stellwagen, N. (1998). DNA gel electrophoresis. Nucleic Acid Electrophoresis Laboratory Manual. (D Tietz, Ed.). Springer Verlag. Berlin-Heidelberg-New York. Roux, K. (2009). Optimisation and troubleshooting in PCR. Cold Spring Harbour Protocols. doi:10.1101/pdb.ip66. Ruano,G.,Pagliaro,E., Schwartz,T.,Lamy,K.,Messina,D.,Gaensslen,R. et al. (1992).Heat-soaked PCR: An efficient method for DNA amplification with applications to forensic analysis.Bio Techniques.13:266ââ¬â274.
Wednesday, November 13, 2019
Bioprospecting :: Geology
Abstract The world's rainforests are host to a multitude of plant and animal species, thus comprising Earth's richest and most diverse natural resource. One of the greatest benefits that rainforests have to offer is its plentiful supply of active compounds, which are used presently in many pharmaceuticals and hold the potential for the next "miracle drug." Bioprospecting is the term used to describe the extraction of natural medicines from our rainforests. Present rates of deforestation, however, pose a serious threat to our "natural pharmacy" and are daily eliminating species of plants that might provide a possible cure. Establishing policies to regulate the bioprospecting industry is challenging, but underway so that humans can benefit from our rainforests while still protecting and conserving the environment. Return to Table of Contents Introduction 2100 AD : It's a horrible situation. Your loved one has been suffering from cancer and there is almost nothing you can do. All possible combinations of drugs have been tried, but still he is suffering from pain. For some reason, medical technology cannot find a way to leave him in peace, allowing him to enjoy his days until he fully recovers. Feeling helpless, you decide to do some research on your own, where you come across a native plant that indigenous peoples have been using for centuries to cure all kinds of pain resulting from various illnesses. This fills you with hope, thinking that you might be able to ease your loved one's pain. Pursuing this line of hope, you consult with some specialists in the medical field, only to become disheartened once again. The plant you read about no longer exists; due to the massive destruction of the rainforests the environment where this plant originated is long gone. 1998: Indigenous peoples have been using the natural resources of our rainforests for centuries to cure a variety of ailments. Medical science is very advanced, yet still cannot duplicate what nature has given us. The simplistic problem illustrated above is very real. Deforestation is not only disrupting ecosystems and wiping out precious animal species, it is also eliminating an enormous potential supply of medicines available in our rainforests. If proper action is not taken today, we will be sacrificing potential "miracle drugs" and losing an extremely valuable source of medicines. Return to Table of Contents What is Bioprospecting? In order to understand bioprospecting, the concept of biodiversity must first be clarified. Biodiversity is defined as "the totality of genes, species, and ecosystems in a region", or "the variety and variability of life.
Monday, November 11, 2019
Financial Crisis of 2008 Essay
The financial crisis is the problem that is faced by many countries like United Nations, Canada and others. It came as a result of economic difficulties triggered by the financial markets, currency fluctuations and liquidity shortfall in banking. The problem started from financial markets to the entire economy. The crisis in 2008 triggered falling of prices for homes. The homes prices in United States dropped after the depression of home sales (William, 2012). There was high rate of unemployment and tighter credit. This led to the drop of real estate prices which encouraged massive increase in construction and extraction of home equity. The housing price affected the recession and economic recovery in United States. United States is recovering slowly from the financial crisis after 2008. The studies show that the financial crisis comes as a result of growth-stifling policies. They clarify their issue in political perspective by showing that in 2012 the voters blamed their political leader especial president Bush for the financial crisis in their country. They still blame president Barrack Obama for the weak recovery (Davis, 2014). According to the research of Kenneth Rogoff and Carmen Renhart, they claim they predicted the financial crisis before it took place. They also claim that their research showed there would be slow recovery. This is seen in how United States experiences slow recoveries and people blaming their leaders for it. The recession took place occurred when the governments, households and corporations were in great debt. This is what triggered a high rate of crisis in finance. The research show that on the issue of crisis Bordo argues that recession tend to lead into strong recovery. He does not concur with Kennetth and Carmeths research. The financial crisis started in 2007 in United States resulted in the federal bank of US investing in financial markets. By the year 2008, the crisis had worsened and the markets around the world had become volatile. Those who had taken up loans were unable to pay for mortgages and borrowers found themselves with negative equity. The other aspects that also led to financial crisis were the collapse of Lehman Brothers (Davis, 2014). Due to their collapse the government was forced to work hard in order to restore its financial institutions. In order to secure itself it had to propose a plan that required around 700 billion dollars. The plan failed due to opposition from people. Instead, people started to invest in gold and US dollar euro as an alternative to save the country from financial crisis since housing market had collapsed. President Obama helped restore United States to its place by spending one trillion dollars. Despite of his effort the people blame him of the slow recovery. According to Lothian & Dwyer (2011), the United States support for current recovery is similar to that of the Great Depression. They do not provide support to link low employment and the high employment in the current recovery. They focus on low aggregate demand and policies that decreases productivity in the country. The research shows that there is a high rate of poverty levels, low birthrates and crippled middle class. This shows that America is still under crisis. According to the president the slow recovery is to the deep recession faced by the country. He claims it will take long time before US recovers. According to Williams (2012) the Federal Reserve has made a lot of effort by taking a step to combat the issue of financial crisis in countries like United States and Canada. It has developed great goals of maximizing employment and make sure there is stable prices. Although the Federal Reserve is doing its best unemployment is still on a high rate. The economic crisis encountered at the early 2000 when the state was still recovering from the recession caused a deep depression in the country. Before the states had recovered full another crisis took place in 2008. The housing market was the only thing that took off to boost the economy of the country in United States. People become wealthier financially. Housing was the main business that boosted the economy of the states financially. The buyers ended up in the housing business in the market with courage of it expanding in future. Acquiring mortgages became much easier and the business mushroomed afterwards (Williams, 2012). The financial engineers took steps of selling mortgages to investors all over the world. The lenders on the other hand emerged and they could sell mortgages with high rate interest. The financial crisis began again when people who had low income just signed up for a mortgage and walked away without making a down payment. This is due to the financial institutions making lots of money at that time since the rise of housing markets had reduced. After the boom on the housing, the financial institutes and lenders encountered great loss which resulted in placing big bets on mortgages. This shows that U.S has not fully recovered and is still doing so slowly due great depression in housing market. The first recovery they conducted was by spending resources on construction and household goods. The Federal Reserve has exerted its effort to recover countries from financial crisis (World Bank, 2010). It innovated the world by setting up Large-scale asset purchases (LSAPs). The LSAPs introduced liquidity to boost the economy through banks purchasing financial assets like mortgages. This helped raise the financial situations in markets not just in united states but worldwide. Their collaboration with the banks helped lower the level of financial assets. The Federal Reserve has done its best to help retain United States to its sustainable economic growth (Williams, 2012). Despite all this efforts the economy of U.S remains weak and inflation below what the Federal Reserve has been targeted. According to Reinhart research, Americans still commit the same mistakes of having weak banks that are unable to make new loans and encourage economic growth. He claims the banks are not performing and may lead to financial crisis again. Some recent studies points out that the financial crisi s may lead to other quick recoveries and studies need to be conducted to focus on how long it will take for the economic standard to return to its level. As for Canada they did not experience high rate of financial crisis as compared to United States. The main problem they encountered was with the Canadian marketing collapsing. Both public and private sectors helped to resolve the situation (Lane & Ontara, 2014). The recession that occurred in Canada led to collapsing of exports. This did not affect Canada alone but also United States since a half of its products were exported to United States. It encountered great loss since both countries were facing financial crisis. Their business was linked to economic investments and housing that collapsed due to recession in the country and United States (Lane & Ontara, 2014). To save itself from economic decline it initiated monetary policy to expand Canadian households and companies to rise to its level. Canada made sure that prices for natural resources remain elevated to help its country quickly pick up. This enabled boost its income, employment and government revenue. Canada is among the countries that have quickened its process to recover from financial crisis. In 2010 it had already passed pre-crisis peak and employment was back into track (Lane & Ontara, 2014). Although it picked up quickly than other countries it needs to lay down more excellent strategies to fully recover from financial crisis. Its economic standard is unbalanced which causes problems to financial system. Its efforts of United States to strengthen its export with Canadian did not work. This shows that it is still facing some financial crisis since U.S was the main country it exported its goods to. However, Australia is also among the countries that faced financial problems but it has done its best to combat the problem. Their president delivered a budget in order to fight inflation. He guaranteed bank deposits with 10.4 billion dollars. It enabled pay for care, families and seniors. He also gave help to automotive industry since lenders at that time had withdrawn from the market. Their withdrawal left banks empty. In 2009 they announced another stimuli package of 47 billion dollars to help boost the economy of Australia (Lane & Ontara 2014). These helped built schools, repair roads, boost small business, construction of new homes and to pay taxpayers. Australia among other countries has made a great improvement to its economy and financial state is not at risk. Nevertheless, among the countries that faced global crisis Poland turns out to be a little bit of resistance for a long time. It was not affected by the first crisis. It has made efforts to heighten its credit policies and the banks being re-assessed. Poland is the only country that has raised its economic growth in the first half 2009(Jungmann & Segemann, 2011) due to its minor share in machinery and equipment in exports. The global crisis had little impact on its economic situation. Their economic growths in 2007 only fell for a little rate. It has increased financial due to construction and assembly production. Employment and wages is not an issue since it is of a high rate compared to other countries. Apart from that, their profit of construction business gives positive results (.Jungmann & Segemann, 2011). The financial crisis in 2008 had little impact to its construction growth. It only affected its investment growth and deterioration in financial cooperation. In 2010 the reces sion spread all over in the construction and engineering. Despite of the effects on the sector Poland has shown slight improvement in the sector. This shows how its construction sector still faces little financial crisis. Since 2008 the countries continue to suffer the financial crisis due to the boom that took place in 2008. Lack of oversight and regulation by united states is a major contributions to the crisis which led to the huge costs to investors. The united states remain to be central in the problems of financial crisis. To solve the global financial crisis countries such as China, India and Russia have collaborated with advanced industrial nations of North America and others to combat the financial crisis (Ciro, 2013). The financial crisis has also enabled large currency reserves to assume higher profiles in financial circles. President Obama conducted a congress required to close attention and skills to manage relations with Chinese leadership. This is due to Chinese being a contributing factor to United States Mortgage bubble (Ciro, 2013). United state still faces financial crisis due to having many trade crisis. Trade is very important it is able to reduce financial vulnerabilities and red uce financial shock to countries. The main reason why United States still faces the financial risks is due to lack of tires with its traders. The only country that did not suffer from high rate of financial crisis in 2008 is china. It is the only country that has brought recovery in many countries. It has played a big role of a main engine in restoring economy in the world. According to the World Bank (2010) china still maintains high percentage in growth. The global economy is still recovered slowly. This has forced china as country with its expanding market to shift from export oriented to domestic- pull (World Bank, 2010). China economics has improved becoming the second biggest growth engine. The World Bank explains out that the financial crisis in many countries have reduced due to the prices in the economy strength. Countries like Korean have difficulties in combating the problem of financial crisis. Their government policies have been exhausted creating difficulties to remedy the financial crisis (Lin, 2013). Apart from that, South Africa has picked up since the financial crisis economy has improved. This is due to the hosting of world cup in 2010. Their banking system has remained stable. They have been introduced diversification by building manufacturing industries to address unemployment crisis (Lin, 2013). As to Brazil the economic situation is unsteady but with the large capital inflows and taxes imposed on foreigners, Brazil is trying to recover from the crisis. The financial crisis continues to be a problem due to epidemics such as viruses, and the gap between countries that perform best and worse in trade. Many business people are missing out on opportunities in the markets to expand their growth. There is marginalization and lack of unemployment to teenagers. House prices are still a major problem and due to this many people are left homeless. Despite of all of this has, globalization has helped incur the financial crisis that took place (Bann, 2014). The globalization has boosted the trade system and in future many countries will recover fully and gains trillions. The international interaction has boosted the economy since people are able to do businesses together. Globalization has worked as a connection index to link people together in boosting trade and export systems in markets (Bann, 2014). Many countries such as china and United States have linked together to do businesses. This has enabled fight the problem of financial crisis in many countries. Globalization has led to negotiations among bilateral and regional trade which has facilitated access to markets. Some countries are able to access different markets and acquire large market share through exports. The large market share increases their financial levels. The countries mentioned have encountered financial crisis and still are going through the same problem. This is due to the boom that took place in 2008. Many countries have not recovered and they are doing their best to get back to track. The only country that remains strong is china due to its stability in economic growth. It is the only country that tried to resist financial crisis unlike other countries. Countries like Canada and United States continue to face financial crisis and their recovery is slow. This comes as a result of aspects such as unemployment, poverty and reduction in house marketing business. They face trade crisis which comes as a result of their trade with other markets collapsing. They are unable to manage financial crisis due to trade. The recession affected main businesses that boosted the economy revenue of many countries. Accumulation imbalances in financial sectors need to be reduced and provide equality to all areas to enable fight for financial crisis. In order to survive in the market and boost the countries revenue, economies should be vulnerable to external stocks. They should encourage higher growth rates and political risks should be involved when unemployment rate still remain at a high level. After the normal recession that took place earlier the economy still remains the same as it was and recoveries have lost ground. Research on how the crisis will be solved shows that it will take a long time since the process will be slow. People need to take action but not rely on leaders to bring change. Everyone possesses entrepreneurial spirit. This is what is needed to help boost the economic standards of many countries. The entrepreneurs need to sustain their families and businesses to help boost the economic growth of the countries. Conclusion Although the researchers claim the recession brings a quick recovery, it cannot solve the issue of financial crisis. Once a country has fallen into deep depression it takes time and efforts to recover from the incidences, it cannot take a short period neither can the country recover quickly. Recovering from financial crisis needs time thatââ¬â¢s why many countries are still facing financial crisis. Leaders cannot be a solution for the problem in the state, but the help of other people is also required. Countries working together will help in boosting the financial revenue of their states. Since, the financial crisis has not been recovered yet, it will take time for countries like United States to do so. References Bonn (2014) globalization is recovering financial crisis, DHL global connectedness index reveals Ciro, T. (2013) the global financial crisis: triggers responses and aftermath. Print. Davis, J. (2014) Global financial crisis- what caused it and how the world responded. Retrieved on 2th December 2014 Jungmann, J., & Sagemann, B. (2011). Financial crisis in Eastern Europe: Road to recovery. Wiesbaden: Gabler. Lin, C. Y.-Y. (2013). National intellectual capital and the financial crisis in Brazil, Russia, India, China, Korea, and South Africa. New York: Springe Lothian, J.R. & Dwyer, P.G (2011) the financial crisis and recovery . Retrieved on 2th December 2014. M.C.K (2012). Is the slow recovery unsual? Retrieved on 2th 2014. Timothy, L. & Ontara, O. (2014) are we there yet? The united states and Canada after the global financial crisis. Retrieved on 2th December 2014 William, C.J. (2012) the federal reserve and the economic recovery. Retrieved on 2th December 2014 World Bank (2010). Global prospets 2010: crisis, finance and growth. Source document
Saturday, November 9, 2019
High Schools do not adequately prepare students for college
High Schools do not adequately prepare students for college While in high school, you are required to take basic level classes to prepare you for your upcoming adult life. High schools do not adequately prepare students for college. Too many students in classrooms leave the slower learners to fall behind. Not enough time in each class forces teachers to skip important topics. Some teachers do not motivate their students to learn.Too many students in classrooms leave the slower students to fall behind. There are not enough teachers for each class to be small, which would give them more one on one time with their students. Many students cannot keep up with the fast paced learning which leaves them to ultimately fail the class or barely pass. When I was in school, we had one teacher to approximately 35 students. There were so many of us asking questions, the teacher could never finish what he/she had planned to cover for that day.Betonwerksteinskulptur "Lehrer-Student" von Reinha...Not enough time in each class forces teachers to skip important topics. In school today, there are seven classes broken up within an eight-hour day. Teachers are forced to highlight over topics that really should be gone over thoroughly. Students do not learn enough in each class when they are only approximately one hour long. When I was in school, we had six periods. Teachers were a little more thorough with each lesson. Small details that are overlooked or deliberately skipped deprive a student of important information.Some teachers do not motivate their students. There are some teachers who sit back and let the students goof off during their classes. This not caring attitude spills over to the students. One teacher I had in school would sit with his feet propped on his desk and would hand out assignments for us to complete. The only problem...
Wednesday, November 6, 2019
Robert Hayden essays
Robert Hayden essays Robert Hayden, the twentieth century poet, wrote poems that many times used history and form that allowed him to craft narratives that had different voices. He used historic themes in many of his poems to show the shared heritage of the African Americans. He also used form to get his message across, invoking a sense of speed, ideas, or to use different voices to focus the poem through. His poems Night, Death, Mississippi, and Runagate, Runagate, both showcase his use of history and the ways he used form in his poetry. In his poem Night, Death, Mississippi Hayden writes about the lynching a black man late at night somewhere in Mississippi. This is a historic poem, written in a grisly but effective manner. It is historically accurate in telling the story of a lynching in the details. The white men coming at night, the white robes, and the details of the lynching are all historic, and true things. The lynching in the poem is unspecific, as it could be any one of hundreds that have occurred in the south since the Civil War. The poems main theme or idea, is to show how completely inhuman blacks were thought to be. In the first part of the poem, an old white man sits on the porch in the darkness, listening to the cry of the lynched black man. He is sick and too weak to go out with the lynching party, but he wishes he were out there. He also remembers fondly the time he had cut of the genitals of a black man and listened to him scream in pain. The second part of the poem focuses on the son, and he tel ls the details of the lynching and why he enjoyed better then hunting animals. All of this serves to show the actual feelings many whites had or still have about blacks mainly in the south, but they exist elsewhere too. The poem Night, Death, Mississippi starts with the lines A quavering cry. Screech owl? Or one of them? Right of the start, the poem brings a sense that something is not right. It is not till the second stanza th...
Monday, November 4, 2019
Strategic Marketing Management Essay Example | Topics and Well Written Essays - 500 words
Strategic Marketing Management - Essay Example However, it should be noted that portfolio analysis tools should be applied with caution as each of the individual technique has limitations. These are further elaborated on the discussion of the different tools. The three main portfolio planning tools are The Boston Consulting Group Growth-share Matrix, General Electric Market Attractiveness Competitive Position Model, and Shell Directional Policy Matrix. The Product Life Cycle (PLC) as a portfolio analysis tool highlights four stages in a product's life cycle-introduction, growth, maturity and decline. This technique stresses that products life is limited and each stage in the life cycle offers different levels of potential gains. Thus, companies should employ the right strategies to maximize cash flow. PLC is valuable as a marketing tool because it emphasizes product termination, growth projections, different marketing objectives and strategies in each stage, product planning, and dangers of overpowering. However, it is also recognized that PLC is limited as each product follows a unique life cycle (e.g., fads and classics), PLC is the result of marketing efforts and not the cause, the time span of each stage is unpredictable, and misleading objectives and strategies. The PLC can always be a good tool choice for compani
Saturday, November 2, 2019
Can computer technology improve the lives of people in the developing Essay
Can computer technology improve the lives of people in the developing world - Essay Example In a developing country, usually the infrastructure is not very strong and people find it difficult to travel from one place to another. This usually creates the urban-rural divide and people in the rural areas are often unable to receive better facilities. With the use of computer technology and internet, the patients in the rural areas can consult the doctors in the cities via video conferencing. In some cases, the doctors can even assist in surgeries at rural locations via video conferencing. This video conferencing could also help the doctors in developing countries to connect to doctors in developed countries and seek their advice and support. In developing countries, the internet becomes a powerful means of communication, especially amongst the youth. It serves to connect the youth together. Through this internet, governments and health agencies can disseminate important information related to health to the young population. This includes information on HIV or several other diseases. This can help in making the people more aware and preventing the spread of these diseases. Normally, developing countries usually face lots of disasters, either manmade or natural. This includes earthquakes, wars and other such calamities. There is lots of damage to life and the victims of these disasters are often in need of blood. Computer technology can enable the government or independent organizations to develop a database of blood donors, and in case of an emergency, these people can instantly be contacted. This will definitely help in saving plenty of lives. Lieberman, D. (n.d.). The Computers Potential Role in Health Education . Retrieved December 15, 2009, from Informaworld:
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